Identification of Burkholderia pseudomallei in the environment of patients with melioidosis in Tamil Nadu, India

Environmental surveillance of Burkholderia pseudomallei in India is limited, with sparse data from non-coastal regions. This study aimed to detect B. pseudomallei in soil collected near the residences of culture-confirmed melioidosis patients in southern India using molecular methods. A total of 50 soil samples were collected from a depth of 30 cm around the homes of five melioidosis patients in Sivagangai district, Tamil Nadu. Soil DNA was extracted and analyzed using conventional PCR targeting the TTS1 gene cluster of B. pseudomallei. Two(4 %) of 50 soil samples tested were positive for B. pseudomallei by PCR.

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Publication Details

Journal
Indian Journal of Medical Microbiology
Published
2025-10-25
DOI
https://doi.org/10.1016/j.ijmmb.2025.101004
Primary Topic
Burkholderia infections and melioidosis
Type
article
Field-Weighted Citation Impact
0.00
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article

Identification of Burkholderia pseudomallei in the environment of patients with melioidosis in Tamil Nadu, India

Vithiya Ganesan, R. Karthikeyan, Dinesh Rajendran, R. Haribalaganesh et al.
Indian Journal of Medical Microbiology
Burkholderia infections and melioidosis
article

Identification of Burkholderia pseudomallei in the environment of patients with melioidosis in Tamil Nadu, India

Vithiya Ganesan, R. Karthikeyan, Dinesh Rajendran, R. Haribalaganesh, Mundamoole Pavithra, Shyama Devi, Movva Srividya, M. Shagana
article en

Abstract

Environmental surveillance of Burkholderia pseudomallei in India is limited, with sparse data from non-coastal regions. This study aimed to detect B. pseudomallei in soil collected near the residences of culture-confirmed melioidosis patients in southern India using molecular methods. A total of 50 soil samples were collected from a depth of 30 cm around the homes of five melioidosis patients in Sivagangai district, Tamil Nadu. Soil DNA was extracted and analyzed using conventional PCR targeting the TTS1 gene cluster of B. pseudomallei. Two(4 %) of 50 soil samples tested were positive for B. pseudomallei by PCR.

Indian Journal of Medical MicrobiologyVol. 58
Velammal Medical College Hospital and Research Institute (IN), All India Institute of Medical Sciences Bhopal (IN), All India Institute of Medical Sciences (IN)
Openalex Percentile: Top 59%
Burkholderia infections and melioidosis
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Identification of Burkholderia pseudomallei in the environment of patients with melioidosis in Tamil Nadu, India — Vithiya Ganesan, R. Karthikeyan, et al. · Indian Journal of Medical Microbiology (2025) | TGRS Research Map | TGRS