The antibiofilm effects of the dichloromethane fraction of Sideroxylon obtusifolium T. D. Penn on Candida spp.

To analyze the phytochemical profile of the dichloromethane fraction (FDIC) of the leaves of Sideroxylon obtusifolium T. D. Penn and determine its antifungal and antibiofilm activity. Materials and Methods: Phytochemical characterization was performed by qualitative and quantitative screening and Nuclear Magnetic Resonance. Antimicrobial activity was determined by Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Antifungal activity on the growth kinetics (Time-Kill Curve) of Candida albicans was measured. Finally, the prescription and metabolism of C. albicans and Candida glabrata monospecies and mixed biofilms treated with FDIC were determined. Data were analyzed by one-way ANOVA and Tukey's post-test (α = 0.05). Results: Phytochemical characterization suggested a greater presence of saponins and phenolic compounds. FDIC showed MIC values ​​between 250-1000 µg/mL and MFC values ​​between 1000 and >1000 µg/mL. FDIC showed fungicidal activity for 24 h against C. albicans and disrupted the biofilm formation of Candida spp. Conclusion: FDIC showed renewed antifungal activity against adhesion and biofilm formation of Candida spp.