Evaluation of the Bactericidal Effects of Agrimonia eupatoria and Nepeta crispa Extracts on Indicator Bacteria

Background:The increasing prevalence of drug-resistant pathogens has drawn researchers' attention to studies on the antimicrobial activity of various substances.This study investigates the antimicrobial effects of the Agrimonia eupatoria and Nepeta Crispa extracts, which are used as herbal medicines.Methods: The study investigated the effects of extract concentrations of 10, 20, and 40 mg/mL on three distinct bacterial strains, encompassing both types of Gram-positive and Gram-negative bacteria, including Escherichia coli, Shigella, and Clostridium perfringens.The evaluation employed the well diffusion method, supplemented by the determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results:The results showed that the Nepeta Crispa extract exhibited no antimicrobial effects at various concentrations, whereas the Agrimonia eupatoria extract was effective against all three types of bacteria, with the diameter of the inhibition zones increasing with higher concentrations.At a concentration of 40 mg/mL, the diameter of inhibition against the three types of bacteria was approximately 8 mm.The Minimum Inhibitory Concentration for Escherichia coli and Shigella was 2.5 mg/mL, and for Clostridium perfringens, it was 1.25 mg/mL.The Minimum Bactericidal Concentration for Escherichia coli, Shigella, and Clostridium perfringens was found to be 2.5 mg/mL, 5 mg/mL, and 2.5 mg/mL, respectively. Conclusion:The extracts of some plants, such as Agrimonia eupatoria have antibacterial properties, but their usage of them needs more investigation.