The effect of guide RNA thermal denaturation on the quality of Cas9 ribonucleoprotein-loaded lipid nanoparticle formulations
Gene-editing technology uses a system that delivers CRISPR/Cas 9 as ribonucleoproteins (RNPs) in a faster and more transiently effective manner than other delivery methods that involve gene expression.
Authors
- Yuji Kashiwakura (ORCID: https://orcid.org/0000-0003-0585-3937)
- Hideyoshi Harashima (ORCID: https://orcid.org/0000-0002-1568-9547)
- Manabu Tokeshi (ORCID: https://orcid.org/0000-0002-4412-2144)
- Tsukasa Ohmori (ORCID: https://orcid.org/0000-0001-5082-6394)
- Katsumi Maenaka (ORCID: https://orcid.org/0000-0002-5459-521X)
- Yusuke Sato (ORCID: https://orcid.org/0000-0003-0913-7815)
- Yuma Yamada (ORCID: https://orcid.org/0000-0002-4637-5102)
- Morisada Hayakawa (ORCID: https://orcid.org/0000-0002-1869-2450)
- Rina Shimizu
- Masatoshi Maeki (ORCID: https://orcid.org/0000-0001-7500-4231)
- Mina Sato
Institutions
- Jichi Medical University (JP)
- Hokkaido University (JP)
- Hokkaido Pharmaceutical University (JP)
Publication Details
- Journal
- RSC Pharmaceutics
- Published
- 2025-10-27
- DOI
- https://doi.org/10.1039/d5pm00189g
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Hokkaido University
- Japan Society for the Promotion of Science