Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass
These findings show that C. thermocellum is unable to effectively degrade RG-I pectic and galactomannan hemicellulosic components in switchgrass biomass. Targeting these polymers for improved solubilization could enhance the efficiency of conversion of grass biomass to biofuels.
Authors
- Neal N. Hengge
- Yannick J. Bomble (ORCID: https://orcid.org/0000-0001-7624-8000)
- Ian Black (ORCID: https://orcid.org/0000-0001-7075-7517)
- Xiaochun Cheng (ORCID: https://orcid.org/0000-0003-0371-9646)
- Nancy L. Engle (ORCID: https://orcid.org/0000-0003-0290-7987)
- Ajaya K. Biswal (ORCID: https://orcid.org/0000-0002-2643-155X)
- David Ryno
- Sushree S. Mohanty
- Melani A. Atmodjo (ORCID: https://orcid.org/0000-0001-5176-0008)
- Debra Mohnen (ORCID: https://orcid.org/0000-0001-5249-635X)
- Parastoo Azadi (ORCID: https://orcid.org/0000-0002-6166-9432)
- Paul E. Abraham (ORCID: https://orcid.org/0000-0003-2685-9123)
- Timothy James Tschaplinski (ORCID: https://orcid.org/0000-0002-9540-6622)
Institutions
- Oak Ridge National Laboratory (US)
- National Laboratory of the Rockies (US)
- University of Georgia (US)
- Duke University (US)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-10-27
- DOI
- https://doi.org/10.1186/s13068-025-02703-9
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Energy
- Office of Science
- Basic Energy Sciences
- Biological and Environmental Research
- Center for Bioenergy Innovation