AAV-mediated delivery of a proliferation inducing ligand (APRIL) to cortical neurons limits inflammation and demyelination in the corpus callosum of the cuprizone mouse model
AAV-mediated expression of APRIL by cortical neuron counteracted microglial recruitment and demyelination in the splenium of CPZ-treated mice. Our multi-omics integration analysis nominated several pathways, including Fabp7-linked astrocytic lipid metabolism, to elucidate APRIL's mechanism of action. Together, these findings provide a framework for the mechanistic dissection of APRIL's therapeutic potential and further translational evaluation in CNS pathologies characterised by acute neuro-inflammatory responses.
Authors
- Damiana Pieragostino (ORCID: https://orcid.org/0000-0003-1015-3484)
- Esther Bartholomeus (ORCID: https://orcid.org/0000-0003-2127-5161)
- Vincenzo De Laurenzi (ORCID: https://orcid.org/0000-0002-7506-1743)
- Bertrand Huard (ORCID: https://orcid.org/0000-0003-1358-6576)
- Marleen Verhoye (ORCID: https://orcid.org/0000-0002-6267-0611)
- Eva Lion (ORCID: https://orcid.org/0000-0002-2420-0301)
- Yousra El Ouaamari (ORCID: https://orcid.org/0000-0003-4075-2445)
- Una FitzGerald (ORCID: https://orcid.org/0000-0002-8019-6546)
- Piero Del Boccio (ORCID: https://orcid.org/0000-0003-1653-2194)
- Federica Di Marco (ORCID: https://orcid.org/0000-0002-1281-607X)
- Peter Ponsaerts (ORCID: https://orcid.org/0000-0002-1892-6499)
- Nathalie Cools (ORCID: https://orcid.org/0000-0002-0459-901X)
- Johan Van Audekerke (ORCID: https://orcid.org/0000-0001-5944-4640)
- Julia Di Stefano (ORCID: https://orcid.org/0000-0003-3825-9803)
- Steven Van Laere (ORCID: https://orcid.org/0000-0002-2640-0040)
- Carole Faghel
- Ilaria Cicalini (ORCID: https://orcid.org/0000-0002-9792-2225)
- Nicholas Vidas-Guscic (ORCID: https://orcid.org/0000-0001-7467-5367)
- Joëlle van Rijswijk (ORCID: https://orcid.org/0000-0001-6971-7358)
- Leonardo Ricciardi
- Ignace Van Spilbeeck
- Tejaswini Kakunuri
- Alexandro A. Bufi
- Marion Decrop (ORCID: https://orcid.org/0009-0005-7745-8188)
Institutions
- Università Cattolica del Sacro Cuore (IT)
- University of Antwerp (BE)
- Science Foundation Ireland (IE)
- Ollscoil na Gaillimhe – University of Galway (IE)
- University Hospital Galway (IE)
- University of Chieti-Pescara (IT)
- Infectious Diseases Institute (UG)
- Translational Innovation in Medicine and Complexity (FR)
- COPE Galway (IE)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Journal of Neuroinflammation
- Published
- 2025-10-27
- DOI
- https://doi.org/10.1186/s12974-025-03569-2
- Citations
- 2
- Primary Topic
- Neurogenesis and neuroplasticity mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 2.64
Funders
- European Commission
- Fonds Wetenschappelijk Onderzoek
- Universiteit Antwerpen
- Vlaamse regering