Multiplex detection and application of MALDI-TOF NAMS for porcine diarrheal pathogens

Porcine diarrheal diseases are a major threat to the swine industry, often caused by multiple viruses and bacteria infecting animals at the same time. Fast and accurate detection of these pathogens is crucial to prevent outbreaks, reduce economic losses, and protect public health, especially given the potential of some pathogens to infect humans. This study introduces a new method that uses nucleic acid mass spectrometry technology to quickly and accurately detect eight major pathogens from pig samples, such as feces, blood, or tissue. Unlike traditional tests that often detect one pathogen at a time, this method can screen for many at once, even in complex cases of mixed infections. It is sensitive, reliable, and can handle large numbers of samples efficiently. This tool offers farmers, veterinarians, and disease control agencies a faster and more effective way to monitor pig health and respond to outbreaks before they spread.