Protection against H9N2 challenge in chickens via <i>Salmonella</i> with a <i>focA</i> mutation delivered by a dendritic cell-targeted avian influenza virus NA protein tetramer
A <i>focA</i> gene deletion was introduced into <i>Salmonella</i> with delayed lysis, resulting in increased cellular escape and colonization. Afterward, a chicken DC-targeting peptide was ligated to the tetramer neuraminidase protein of the H9N2 influenza virus. The combination of the <i>focA</i> gene mutation and chicken DC targeting effectively enhanced the humoral, cellular, and mucosal immune responses, yielding improved protection against H9N2 challenge in chickens.
Authors
- Futing Jia
- Junjie Gong (ORCID: https://orcid.org/0009-0000-4613-7692)
- Chongbo Ge
- Haibin Huang
- Yanlong Jiang (ORCID: https://orcid.org/0000-0001-5843-4301)
- Gerui Zhang (ORCID: https://orcid.org/0000-0003-3191-5476)
- Yupeng Gao
- Zhannan Wang (ORCID: https://orcid.org/0009-0003-6365-612X)
- Yuhang Zhang (ORCID: https://orcid.org/0000-0002-3562-5401)
- Mingyue Wang (ORCID: https://orcid.org/0000-0001-9370-3896)
- Yan Sun (ORCID: https://orcid.org/0000-0003-2782-8687)
- Wenfeng Wang (ORCID: https://orcid.org/0000-0003-4083-4344)
- Yuxi Zhang (ORCID: https://orcid.org/0000-0001-5368-1346)
- Qiyu Guo
- Tongyu Zhang (ORCID: https://orcid.org/0000-0003-0431-5387)
- Chunfeng Wang (ORCID: https://orcid.org/0009-0001-9661-9005)
- Yawen Tian
- Yingkai He
- Tianrui Yang
Institutions
- Health Commission of Jilin Province (CN)
- Jilin Agricultural University (CN)
- Changchun University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-10-27
- DOI
- https://doi.org/10.1128/spectrum.01421-25
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00