Thermostable and recyclable <i>Candida antarctica</i> lipase B immobilized on <i>Bacillus subtilis</i> using sortase technology

This work presents a groundbreaking advancement in biocatalysis, addressing critical challenges in enzyme stability, reusability, and sustainability for industrial applications. This work directly addresses the industrial bottlenecks of enzyme instability, high turnover costs, and environmental impact. The sortase-mediated system offers a universal platform for displaying diverse enzymes, enabling tailored biocatalysts for sectors ranging from bioremediation to synthetic biology.