Poly(ethylene glycol)-graft-Hyaluronic Acid Hydrogels for Angiogenesis

Hyaluronic acid (HA) hydrogels are promising biomaterials for tissue engineering and drug delivery due to their biocompatibility and biodegradability. The objective of this study was to develop a novel HA-based hydrogel for the controlled release of basic fibroblast growth factor (bFGF) to promote angiogenesis. A series of PEG-grafted HA hydrogels with varying PEG grafting ratios were synthesized and characterized. We evaluated their physicochemical properties, including swelling ratio, cross-linking density, and enzymatic degradation behavior, and assessed their ability to control bFGF release and induce angiogenesis in a mouse model. The results showed that the PEG-grafting ratio significantly affected the gel properties. Notably, the PEG60-<i>graft</i>-HA hydrogel exhibited a higher swelling ratio and more rapid degradation, suggesting a non-uniform and highly porous structure. In vitro release studies confirmed that while PEG5-<i>graft</i>-HA and PEG15-<i>graft</i>-HA gels showed burst release, the PEG60-<i>graft</i>-HA hydrogel demonstrated sustained release of bFGF over time. Furthermore, in vivo experiments revealed a significant increase in angiogenesis with the PEG60-<i>graft</i>-HA hydrogel, likely due to the prolonged release of active bFGF. These findings suggest that PEG-grafted HA hydrogels, particularly those with a higher PEG grafting ratio, are promising biomaterials for the controlled release of growth factors and applications in tissue regeneration.