Correlative microscopy for in-depth analysis of calcium oxalate crystals in plant tissues

The presented correlative approach preserved the structural integrity of crystals and cellular structures during cutting and transferring between microscopes. Analysing exactly the same sample (position) by Raman, polarized light microscopy and SEM opens the view on the distribution within tissues and cells as well as the molecular structure of the crystals and adjacent cell structures. Such a comprehensive in-situ characterization paves the way for a better understanding of mineralization processes of different minerals in all kinds of biological tissues.