Characterization of LTR Retrotransposon Reverse Transcriptase in Tamarix chinensis L. and Activity Analysis Under Salt and Alkali Stresses

Transposable elements (TEs) are major components of plant genomes and play crucial roles in adaptive genome evolution and stress tolerance. Under abiotic stress, activated TEs can generate abundant genetic variation and regulate the expression of stress-responsive genes. As a pioneer species in desert and saline-alkali environments, <i>Tamarix chinensis</i> L. has been little studied with respect to the abundance and evolutionary relationships of its LTR retrotransposons, particularly their activation patterns under salt and alkali stresses. This study aimed to investigate the characteristics of the reverse transcriptase (RT) domain of LTR retrotransposons in <i>T</i>. <i>chinensis</i> and to determine their patterns of activation in response to salt and alkali stresses. A total of 629 Ty1-<i>copia</i> and 607 Ty3-<i>gypsy</i> RT nucleotide sequences, which displayed high AT/GC ratios and evidence of stop codon insertions, were identified in <i>T</i>. <i>chinensis</i> by amplicon sequencing. Among these, 211 Ty1-<i>copia</i> and 117 Ty3-<i>gypsy</i> RT sequences with potential transpositional activity each contained distinct domains, suggesting a high degree of conservation. Phylogenetic analysis revealed that the RT sequences of <i>T</i>. <i>chinensis</i> are closely related to those of mangrove, wild potato, and <i>Ipomoea</i>, and may have undergone horizontal transfer. Expression analysis showed that 634 and 181 RT sequences were activated under salt and alkali stresses, respectively, with the majority belonging to salt-induced Ty1-<i>copia</i> families. Compared with the control group, under salt and alkali stresses, the cTy1-<i>copia</i> elements (Ty1-<i>copia</i> with amplificated from cDNA of <i>T</i>. <i>chinensis</i>, the same below) with dominant abundance were mainly concentrated in the Angela subfamily, while the cTy3-<i>gypsy</i> elements induced by alkali stress were primarily distributed in the Tekay and Reina subfamilies. Furthermore, four cTy1-<i>copia</i> and five cTy3-<i>gypsy</i> were identified as candidate key LTR retrotransposons responsive to salt and alkali stresses. Overall, this study provides new insights into the epigenetic mechanisms underlying the adaptation of <i>T</i>. <i>chinensis</i> to saline and alkali stresses and offers a theoretical basis for its potential applications in saline-alkali land reclamation.