Exosome-Based Compound Activates Skin’s Keratinocytes and Fibroblasts

Introduction: Exosomes have emerged as promising agents in cosmetic science due to their ability to stimulate skin regeneration, collagen synthesis, and anti-aging processes. This study aims to evaluate the biological effects of Exocoll, an exosome-based compound, on cellular energy, wound healing, and the expression of anti-aging genes in human keratinocytes and fibro-blasts. Methods: Human keratinocytes and fibroblasts were cultured and treated with Exocoll. Cellular energy was evaluated using the sulforhodamine B assay. Wound healing was assessed via scratch assays, and the expression of anti-aging markers (FoxO3, SIRT-1, and SIRT-3) was analyzed using real-time PCR. Results: Exocoll significantly enhanced mitochondrial activity in keratinocytes by 13%. In scratch assays, treated keratinocytes achieved 99.9% wound closure within 24 hours, compared to 68.5% in the control group. In fibroblasts, Exocoll upregulated the expression of FoxO3 by 84.6%, SIRT-1 by 44.8%, and SIRT-3 by 52.1%. Discussion: These findings indicate that Exocoll stimulates key pathways associated with skin repair and longevity. The enhanced energy metabolism and upregulation of anti-aging genes sug-gest mechanisms consistent with improved cellular function and regenerative capacity. While the results are promising, further investigations, including in vivo studies, are needed to confirm ef-ficacy and safety in clinical applications. Conclusion: Exocoll effectively stimulates cellular energy, promotes anti-aging gene expression, and accelerates wound healing, making it a promising candidate for anti-aging skincare and re-generative treatments. conclusion: Exocoll effectively stimulates cellular energy, promotes anti-aging gene expression, and accelerates wound healing, making it a promising candidate for anti-aging skincare and regenerative treatments.