The Myo2 adaptor Ldm1 and its receptor Ldo16 mediate actin-dependent lipid droplet motility
Organelle motility enables strategic cellular reorganizations. In yeast, this process depends on the actin cytoskeleton, type V myosin motor proteins, and organelle-specific myosin adaptor proteins. While the myosin adaptors for most organelles are known, the coupling of myosin to lipid droplets (LDs), the cellular lipid storage organelles, remained enigmatic. Using genome-wide screening, we identified Ldm1 (lipid droplet motility 1/Yer085c) as a myosin adaptor. Ldm1 binds to the globular tail domain of the myosin Myo2 and to the LD surface protein Ldo16 to enable actin-dependent LD motility. Ldo16 has additional roles in LD contact sites to the vacuole and the endoplasmic reticulum, suggesting a coordination of LD motility and organelle tethering. Ldm1 has a second role in mitochondrial transport, and elevated Ldm1 levels rescue defects of the mitochondrial Myo2-adaptors Mmr1/Ypt11. Our work identifies the molecular machinery for LD motility and contributes to a comprehensive understanding of acto-myosin-based cellular reorganization.
Authors
- Barbara R. Leite (ORCID: https://orcid.org/0000-0003-4440-1298)
- Javier Collado (ORCID: https://orcid.org/0000-0002-8707-6619)
- Mike Wälte
- Christian Schuberth
- Marie Hugenroth (ORCID: https://orcid.org/0000-0003-2499-7274)
- Meryem Aysenur Turhan
- Jenny Keller
- Duy Trong Vien Diep (ORCID: https://orcid.org/0000-0002-8665-3488)
- Roland Wedlich‐Söldner (ORCID: https://orcid.org/0000-0002-1364-7589)
- Rubén Fernández‐Busnadiego (ORCID: https://orcid.org/0000-0002-8366-7622)
- Thomas Becker (ORCID: https://orcid.org/0000-0002-1138-5263)
- Daniel Kümmel (ORCID: https://orcid.org/0000-0003-3950-5914)
- Florian Fröhlich (ORCID: https://orcid.org/0000-0001-8307-2189)
- Rebecca Martina Fausten (ORCID: https://orcid.org/0000-0001-8726-4970)
- Maria Bohnert (ORCID: https://orcid.org/0000-0002-8154-555X)
- Bianca M. Esch (ORCID: https://orcid.org/0000-0002-3645-3322)
- Stephan Wilmes (ORCID: https://orcid.org/0000-0003-1084-2300)
- Pascal Höhne (ORCID: https://orcid.org/0009-0007-1128-2110)
- Louis Percifull
- Xue-Tong Zhao
Institutions
- University of Bonn (DE)
- Osnabrück University (DE)
- University of Münster (DE)
- Universitätsmedizin Göttingen (DE)
- FH Münster University of Applied Sciences (DE)
- University of Göttingen (DE)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-10-25
- DOI
- https://doi.org/10.1016/j.celrep.2025.116475
- Citations
- 2
- Primary Topic
- Nuclear Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 1.61