Challenges and caveats in manipulating extracellular vesicle secretion from pancreatic cancer cells

Overall, we found that these models did not perform as anticipated, and we could not consistently inhibit KPC cell EV secretion. These results emphasize the challenges of interfering with EV secretion, as several parallel pathways, such as direct membrane budding, can compensate. Further studies are needed to develop models for studying the role of EVs <i>in vivo</i>.