Protocol for immunodetection of α-synuclein pathology in paraffin-embedded liver tissues from murine models of Parkinson’s disease

The accumulation of α-synuclein (α-Syn) pathology in peripheral tissues of Parkinson's disease (PD) has attracted growing scientific interest in recent years. Here, we present a protocol for the immunodetection of α-Syn pathology in murine liver tissue from models of PD using fluorescence microscopy. We describe steps for liver isolation, fixation, embedding, and immunodetection using an array of antibodies targeting α-Syn. This approach offers valuable applications for studying PD in transgenic mouse models and could be adapted for human liver tissue. For complete details on the use and execution of this protocol, please refer to Hallbeck et al.¹.