Light and Dark Mediated Synthesis of Silver Nanoparticles from <i>Moringa Oleifera</i> Leaves and <i>Myristica Fragans</i> Seeds

ABSTRACT In this study, we have examined and compared the effect of both light and dark on the synthesis of silver nanoparticles(Ag NPs)in water and DMSO using Moringa oleifera leaves and Myristica fragrans seeds. To verify AgNP formation, shape, and stability, the produced nanoparticles were subjected to UV–visual spectroscopy. Further comparative antibacterial broth culture approach was being carried out in order to establish their role as inhibitors. Analysis of the effects of light conditions on the rate of synthesis of AgNPs showed that, in contrast to dark settings, sunlight greatly improved the reaction kinetics. Because of the increased energy availability, exposure to sunlight accelerated the reduction of silver ions to AgNPs, producing smaller and more homogeneous particles. From the UV analysis of AgNPs, sunlight mediated AgNPs and dark mediated AgNPs prepared in water and DMSO at different concentrations, we found that the size of the nanoparticle gets effected. Sunlight induced aqueous Ag NPs from Myristica fragans seeds showed a significant reduction of E.coli growth in broth followed by dark induced Ag NP in DMSO from Myristica fragans . This study highlights the potential of Ag NPs made from plant extracts as an effective and natural way to fight bacterial infections, which may find use in the pharmaceutical, medical, and environmental domains.