Chemodiversity of Cyanobacteria from Brazil Investigated by Metabolomics and Bioassays
Investigations on cyanobacterial secondary metabolites in Brazil have been scarce, despite the country's significant biodiversity. Herein, we report the results of a screening of cyanobacterial cultures using both bioassays and untargeted metabolomics. Nineteen cyanobacterial strains collected at various locations in Brazil were cultured. Cultures were extracted and prefractionated. Fractions were evaluated for antibacterial, cytotoxic, and antileishmanial activities. The same fractions were analyzed by UHPLC-HRMS-MS/MS. Results from bioassays and LC-MS were integrated using metabolomics tools such as NP Analyst and GNPS Molecular Networking, allowing for feature prioritization. Cyanobacteria belonging to genera <i>Calothrix</i> and <i>Phormidium</i> presented high-priority molecular features associated with observed biological activities, indicating that such strains are producers of potentially novel and bioactive metabolites.
Authors
- Michael J. J. Recchia (ORCID: https://orcid.org/0000-0002-1450-0941)
- Roger G. Linington (ORCID: https://orcid.org/0000-0003-1818-4971)
- Camila Manoel Crnkovic (ORCID: https://orcid.org/0000-0001-6581-9031)
- José Ângelo Lauletta Lindoso (ORCID: https://orcid.org/0000-0002-4405-4401)
- Roberto G. S. Berlinck (ORCID: https://orcid.org/0000-0003-0118-2523)
- Helori Vanni Domingos (ORCID: https://orcid.org/0000-0003-3571-2588)
- Cristhiane Gonçalves (ORCID: https://orcid.org/0000-0002-3894-2971)
- Francisco Henrique da Silva (ORCID: https://orcid.org/0000-0003-1264-5597)
- Frederico J. Gueiros‐Filho (ORCID: https://orcid.org/0000-0001-5425-2439)
- Letícia V. Costa‐Lotufo (ORCID: https://orcid.org/0000-0003-1861-5153)
- Sandra Regina Soares (ORCID: https://orcid.org/0000-0003-3343-326X)
- Hannah Cavanagh
- Leonardo Santos de Jesus
- Kleyton J. G. de Morais
Institutions
- Universidade de São Paulo (BR)
- Simon Fraser University (CA)
- Instituto de Infectologia Emílio Ribas (BR)
- Instituto de Medicina Tropical (PY)
- Instituto de Ciências Farmacêuticas (BR)
- Instituto de Psicologia Comportamental de São Carlos (BR)
Publication Details
- Journal
- ACS Omega
- Published
- 2025-10-24
- DOI
- https://doi.org/10.1021/acsomega.5c07322
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00