Impact of tissue storage time on immunodetection of c-Fos and GAD67 in the rat brain
These findings underscore that whereas intact brains can be safely stored for prolonged periods at 4°C without compromising antigenicity, brain slices are highly susceptible to storage-induced deterioration - insights important for planning and interpreting immunohistochemical studies in neuroscience.
Authors
- Marion Inostroza
- Jan Born (ORCID: https://orcid.org/0000-0002-1847-6248)
- Stoyan Dimitrov
- Shan Xia (ORCID: https://orcid.org/0000-0001-9967-0394)
Institutions
- Deutsches Diabetes-Zentrum e.V. (DE)
- German Center for Diabetes Research (DE)
- Heinrich Heine University Düsseldorf (DE)
- University of Tübingen (DE)
Publication Details
- Journal
- Journal of Neuroscience Methods
- Published
- 2025-10-20
- DOI
- https://doi.org/10.1016/j.jneumeth.2025.110602
- Citations
- 2
- Primary Topic
- Cell Adhesion Molecules Research
- Type
- article
- Field-Weighted Citation Impact
- 3.24