Functional assessment of Streptomyces coelicolor secretome proteins associated with biogenic silver nanoparticles exhibiting multiple bioactive effects
Streptomyces coelicolor spent fermentation medium was used to produce biogenic silver nanoparticles (ScoAgNPs) which were characterized for their size, morphology, stability, structure, macromolecular composition, and bioactivities. In particular, ATR-FTIR spectroscopy revealed the presence of lipids, nucleic acids, and proteins in ScoAgNPs. Differential shotgun proteomic analysis - comparing the ScoAgNP protein content with the S. coelicolor secretome - identified proteins accumulated in the biogenic nanomaterial. Bioinformatic analysis revealed their binding domains for nucleotides, carbon compounds, and metal compounds, as well as their predicted molecular functions. Additionally, in vitro assays revealed ScoAgNPs antimicrobial, antibiofilm, anticancer, and seed germination-promoting activities. These properties suggest that the ScoAgNPs could serve as promising bionanotechnological tools with applications in biomedicine and agriculture. Additionally, the identification of associated proteins provides novel insights into the biomolecular mechanisms underlying AgNP biogenesis, also paving the way for rational strategies aimed at optimizing production and enhancing bio-functionality.
Authors
- Miriam Buttacavoli (ORCID: https://orcid.org/0000-0003-4332-8228)
- Ciro Caldiero
- Maria Rosa Abenavoli (ORCID: https://orcid.org/0000-0001-5926-9985)
- Valentina Catania (ORCID: https://orcid.org/0000-0002-9439-0980)
- Giuseppe Gallo (ORCID: https://orcid.org/0000-0003-4727-5653)
- Alessandro Presentato (ORCID: https://orcid.org/0000-0002-4794-0599)
- Roberta Caridi
- Simone Dario Scilabra (ORCID: https://orcid.org/0000-0001-7300-6153)
- Elena Piacenza (ORCID: https://orcid.org/0000-0003-1929-1031)
- Domenico Schillaci (ORCID: https://orcid.org/0000-0003-2416-0474)
- Giorgio Nasillo
- Matteo Calligaris (ORCID: https://orcid.org/0000-0002-5330-3537)
- Teresa Faddetta (ORCID: https://orcid.org/0000-0002-1255-4890)
- Patrizia Cancemi (ORCID: https://orcid.org/0000-0002-3951-7213)
- Delia Francesca Chillura Martino (ORCID: https://orcid.org/0000-0001-5141-7285)
- Serena Indelicato (ORCID: https://orcid.org/0000-0001-9965-3818)
- David Bongiorno (ORCID: https://orcid.org/0000-0002-9856-356X)
- Margot Lo Pinto (ORCID: https://orcid.org/0009-0003-0394-9414)
Institutions
- Institute of Biosciences and Bioresources (IT)
- Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione (IT)
- University of Reggio Calabria (IT)
- University of Palermo (IT)
Publication Details
- Journal
- International Journal of Biological Macromolecules
- Published
- 2025-10-20
- DOI
- https://doi.org/10.1016/j.ijbiomac.2025.148384
- Citations
- 1
- Primary Topic
- Nanoparticles: synthesis and applications
- Type
- article
- Field-Weighted Citation Impact
- 0.52