Histologic evaluation of the effect of grape seed oil and ferric sulfate in pulpotomy applied to molar teeth of rats: An <i>in vivo</i> study.

BACKGROUND: The objective of this study was to examine the healing potential of Grape Seed Oil (GSO) on the pulp tissue that remains following pulpotomy treatment. MATERIALS AND METHODS: The upper first molars of 18 two-month-old male Wistar albino rats were divided into two groups according to the material used in pulpotomy treatment. In the pulpotomy treatment group, GSO was used, while ferric sulfate (FS) was used in the control group. The rats were euthanized at 24 hours, 15 days, and 30 days following treatment. Histopathological evaluation of the samples was conducted using the hematoxylin-eosin staining method. The evaluation was conducted to ascertain alterations in connective tissue, vascular changes (i.e., angiopathic findings), inflammation, and hemorrhagic findings in the pulp. The data obtained in this study were analyzed with the IBM SPSS Statistics Version 21 software program. RESULTS: In the present study, it was observed that the inflammatory response in the GSO group was higher than that in the FS group at 24 hours. The investigation revealed that the vascular response exhibited a higher magnitude in the GSO group compared to the FS group at 24 hours and at day 15. However, this response exhibited a decline at day 30. Despite an increase in hemorrhaging on the 15th day in the GSO group, this phenomenon decreased over time. CONCLUSIONS: It has been observed that GSO is a biocompatible material that can be used as an alternative to FS in pulpotomy treatment.