Sensitive Cloud Point Extraction Method for Determination of Salbutamol Sulfate in Pharmaceutical Dosage Forms

The cloud point extraction (CPE) methodology was utilized to produce a simple, highly sensitive, and environmentally friendly method for preconcentrating and evaluating salbutamol sulfate (SAL) in pharmaceutical dosage forms. Triton X-114 was used as a surfactant to recover the product result from the diazotization coupling between SAL and diazotized 2, 4-dichloroaniline (DCA) by the cloud point extraction process. The product was extracted with non-ionic surfactant micelles and then spectrophotometrically detected at a wavelength of 445 nm. Under ideal conditions, the calibration curve’s linearity (r = 0.9947) ranged from 0.1 to 5.0 μg. mL–1 of SAL. The results showed that the samples had average recoveries ranging from 97.5 to 99.1%, relative standard deviation (RSD) better than 3.5%, and detection and quantification limits of 0.01 and 0.04 μg.mL–1, respectively. Both batch and cloud point extraction procedures (with and without extraction) were used to study the reaction, and a simple comparison of the two approaches was made. The recommended technique proved effective in determining the SAL in pharmaceutical applications.