Two-step purification of elastin-like polypeptide-fusion superoxide dismutase via hydrophobicity and thermoresponsiveness
These results demonstrate that ELP-mediated purification offers a scalable and economical alternative to conventional methods. The combination of foam separation and thermal precipitation minimizes the need for expensive chromatography, making this strategy particularly promising for industrial-scale biotechnological applications.
Authors
- Dongren Zhou (ORCID: https://orcid.org/0000-0002-0080-6982)
- Hao Zhu
- Yu‐Fei Wu (ORCID: https://orcid.org/0000-0002-3970-3999)
- Aixia Huang (ORCID: https://orcid.org/0000-0002-3601-8769)
- Jinping Chen (ORCID: https://orcid.org/0009-0006-7603-1008)
- Weiwei Wang (ORCID: https://orcid.org/0000-0002-0215-9467)
- Yuchen Wang (ORCID: https://orcid.org/0000-0001-8706-9016)
- Yang Zhou (ORCID: https://orcid.org/0000-0001-8159-6835)
- Feng Lin (ORCID: https://orcid.org/0000-0001-9520-0598)
- X. Dong
Institutions
- Jiangsu University (CN)
- Xuzhou Medical College (CN)
- Suzhou University of Science and Technology (CN)
- Soochow University (CN)
- Zhejiang Institute of Freshwater Fisheries (CN)
- Affiliated Hospital of Xuzhou Medical College (CN)
- Ministry of Agriculture and Rural Affairs (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-10-24
- DOI
- https://doi.org/10.3389/fbioe.2025.1695586
- Primary Topic
- Connective tissue disorders research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jiangsu University
- Xuzhou Medical University
- National Engineering Laboratory of Crop Stress Resistance Breeding