Whole Genome Features and Analysis of Antibiotic Resistance Determinants in <i>Pseudomonas aeruginosa</i> Strain CYZ

<b><i>Background:</i></b> <i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that exhibits a strong resistance ability to antibiotics. <b><i>Methods:</i></b> The complete genome of <i>P. aeruginosa</i> CYZ was sequenced using a PacBio RS II system. The functions of all the predicted genes and proteins were classified and annotated using the Clusters of Orthologous Groups of proteins, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. Furthermore, the antimicrobial resistance genes were also analyzed via the Comprehensive Antibiotic Resistance Database, and several types of antibiotics were selected to test the antimicrobial susceptibility. Phylogenetic relationships were investigated using the single nucleotide polymorphisms (SNPs) from 228 clinically isolated <i>P. aeruginosa</i> strains. <b><i>Results:</i></b> The genome size of <i>P. aeruginosa</i> CYZ is 6,382,603 bp and contains 5,807 protein-coding genes, with an average G + C content of 66.44%. Functional genomic analysis via the annotations of the databases identified a total of 499 antimicrobial resistance genes. <i>P. aeruginosa</i> CYZ showed resistance to 14 types of antibiotics. The phylogenetic analysis revealed that <i>P. aeruginosa</i> CYZ was closely related to <i>P. aeruginosa</i> PAO1. The <i>P. aeruginosa</i> strains exhibited no geographical specificity, and the variation in core genome SNPs was nonrandomly distributed. <b><i>Conclusion:</i></b> Our findings give valuable insight into the genetic antimicrobial-resistant features of <i>P. aeruginosa</i> as well as provide a genetic basis for the further study of the phenotype.