First Detection of an Alphaherpesvirus Gene in Humpback Whale Blow Samples Collected Noninvasively Using Unmanned Aerial Vehicles

Viral infections have a significant impact on wildlife health, population dynamics, and ecosystem stability. Studies of cetaceans-key species in marine ecosystems-are challenging for viral infection research, owing to difficulties in collecting conventional biological samples. In this study, unmanned aerial vehicles (UAVs) were used in 2024 to noninvasively sample exhaled breath condensates (blows) from five groups of humpback whales (<i>Megaptera novaeangliae</i>) along the coastline of an island in the Pacific Ocean south of Japan. Comprehensive virome analysis revealed viral sequences related to 39 known virus species across 18 families, including nine that infect mammals. Notably, partial sequences of the <i>UL20</i> gene similar to an alphaherpesvirus previously identified in beluga whales were detected for the first time in the blows from these humpback whales. Our study demonstrates that UAV-based blow sampling is an effective tool for virological surveillance in cetaceans. Moreover, our findings aid in advancing our understanding of the diversity of viruses in marine mammals and supporting the development of noninvasive monitoring strategies that are critical for ensuring the conservation and health of these creatures.