Non‐Enzymatic Generation of Placenta Single Cells From Third Trimester Human Placenta

The placenta is a heterogeneous and complex organ with multiple cell types, posing a challenge for the maternal-fetal medicine field to implement single-cell technologies for a deeper characterization of this essential organ. Several protocols use enzymes to digest the tissue and generate single-cell suspensions, but this approach has several shortcomings, including the loss and reduced viability of cells. In this article, we describe a non-enzymatic approach to generate single-cell suspensions from placental tissue with high yield and viability for single-cell RNA sequencing. © 2025 Wiley Periodicals LLC. Basic Protocol 1: Preparation of single-cell suspension from freshly collected third trimester human placental sections Basic Protocol 2: Visualization and determination of cell viability Alternate Protocol: Counting cells using a hemocytometer.

Authors

Institutions

Publication Details

Journal
Current Protocols
Published
2025-10-01
DOI
https://doi.org/10.1002/cpz1.70237
Primary Topic
Pregnancy and preeclampsia studies
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Non‐Enzymatic Generation of Placenta Single Cells From Third Trimester Human Placenta

Ryoko Baba, Aliyu Salihu, Musa Abubakar Kana, Benedict Anchang et al.
Current Protocols
Pregnancy and preeclampsia studies
article

Non‐Enzymatic Generation of Placenta Single Cells From Third Trimester Human Placenta

Ryoko Baba, Aliyu Salihu, Musa Abubakar Kana, Benedict Anchang, Kalen Ephraim Audu, Abubakar Musa, Zeenat Bello Kudan, Idowu A. Aimola, Hanneda A. Fomukong, Joy U. Ameloko, Joy B. Karma, Peculiar N. Okoro, Abdullahi B. Salau, Solomon Dunason, Habiba H. Abubakar
article en

Abstract

The placenta is a heterogeneous and complex organ with multiple cell types, posing a challenge for the maternal-fetal medicine field to implement single-cell technologies for a deeper characterization of this essential organ. Several protocols use enzymes to digest the tissue and generate single-cell suspensions, but this approach has several shortcomings, including the loss and reduced viability of cells. In this article, we describe a non-enzymatic approach to generate single-cell suspensions from placental tissue with high yield and viability for single-cell RNA sequencing. © 2025 Wiley Periodicals LLC. Basic Protocol 1: Preparation of single-cell suspension from freshly collected third trimester human placental sections Basic Protocol 2: Visualization and determination of cell viability Alternate Protocol: Counting cells using a hemocytometer.

Current ProtocolsVol. 5(10)
Ahmadu Bello University (NG), Kaduna State University (NG), Kaduna Polytechnic (NG), National Institute of Environmental Health Sciences (US), Maitama Sule University Kano (NG), Kano State University of Technology (NG), Bayero University Kano (NG)
Openalex Percentile: Top 62%
Pregnancy and preeclampsia studies
DOISourceOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Non‐Enzymatic Generation of Placenta Single Cells From Third Trimester Human Placenta — Ryoko Baba, Aliyu Salihu, et al. · Current Protocols (2025) | TGRS Research Map | TGRS