Optimisation and application of a T-cell antigen-specific activation assay as diagnostic and treatment-monitoring tool for tuberculosis
We demonstrated that TB-TASA could also be reliably measured in capillary blood collected <i>via</i> fingerprick from IGRA<sup>+</sup> controls and TB patients, achieving an ROC AUC of 0.96 (95% CI 0.9-1) and sensitivity and specificity of 95% and 69%, respectively. Together, these results support the continued development of TB-TASA as a potential tool for diagnosing TB and monitoring treatment responses.
Authors
- Stephanus T. Malherbe (ORCID: https://orcid.org/0000-0002-7563-8231)
- Mark Hatherill (ORCID: https://orcid.org/0000-0003-3491-1809)
- Hadn Africa
- Laura E. Via (ORCID: https://orcid.org/0000-0001-6074-9521)
- Ray Y. Chen (ORCID: https://orcid.org/0000-0001-6344-1442)
- Lungisa Jaxa
- Clifton E. Barry (ORCID: https://orcid.org/0000-0002-2927-270X)
- Ashley Veldsman
- Gerhard Walzl (ORCID: https://orcid.org/0000-0003-2487-125X)
- Humphrey Mulenga (ORCID: https://orcid.org/0000-0002-2030-0751)
- Elisa Nemes (ORCID: https://orcid.org/0000-0003-1662-4961)
- Robert John Wilkinson (ORCID: https://orcid.org/0000-0002-2753-1800)
- Catherine Riou (ORCID: https://orcid.org/0000-0001-9679-0745)
- Simon C. Mendelsohn (ORCID: https://orcid.org/0000-0002-4054-2766)
- Michèle Tameris (ORCID: https://orcid.org/0000-0002-7983-7203)
- Elizabeth Filander
- Thomas J. Scriba (ORCID: https://orcid.org/0000-0002-0641-1359)
- Munyaradzi Musvosvi (ORCID: https://orcid.org/0000-0002-4526-8479)
- Michelle Fisher (ORCID: https://orcid.org/0000-0003-1414-7612)
- Suzette Visagie
Institutions
- South African Medical Research Council (ZA)
- National Institutes of Health (US)
- University of Cape Town (ZA)
- Stellenbosch University (ZA)
- The Francis Crick Institute (GB)
- South African Tuberculosis Vaccine Initiative (ZA)
- National Institute of Allergy and Infectious Diseases (US)
- Wellcome Centre for Infectious Diseases Research in Africa (ZA)
- Imperial College London (GB)
Publication Details
- Journal
- ERJ Open Research
- Published
- 2025-10-23
- DOI
- https://doi.org/10.1183/23120541.01016-2025
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Bill and Melinda Gates Foundation
- Francis Crick Institute
- UK Research and Innovation
- Cancer Research UK
- National Institute for Health and Care Research
- Imperial College London
- Imperial College Healthcare NHS Trust
- South African Medical Research Council
- Universiteit Stellenbosch
- University of Cape Town
- National Institutes of Health
- Medical Research Council
- National Institute of Allergy and Infectious Diseases
- NHLBI Division of Intramural Research
- European and Developing Countries Clinical Trials Partnership