Human Umbilical Cord Mesenchymal Stem Cells Exert Protective Effects in Peritoneal Fibrosis via cGAS-STING Signaling Pathway

Background: Clinical evidence indicates exposure of the peritoneum to non-physiological peritoneal dialysis fluid may result in peritoneal fibrosis(PF).MSCs can repair damage and fibrosis in organs.In our recently research,we have estimated CAS-STING signal transmissoin is closely associated with PF. Methods: HG-induced HMrSV5 EMT and mouse peritoneum fibrosis induced by PF. hUC-MSCs were co-cultured with HMrSV5, and their effects on HG-induced EMT were evaluated using wound healing, Transwell migration, qPCR, and WB,IHC. Role of cGAS-STING pathway was confirmed using the STING agonist DMXAA. Results: cGAS and STING are upregulated in HG-induced HMrSV5 EMT and mouse PF. Co-culture of hUC-MSCs with HMrSV5 suppresses HMrSV5 EMT induced by HG via inhibition of cGAS-STING signaling pathway. Moreover, treatment of PF mice with hUC-MSCs alleviates PF,local inflammation,downregulates proteins of the cGAS-STING signaling pathway , Conclusion: hUC-MSCs mitigate EMT in HMrSV5 and retard the progression of PF in mice through inhibition of the cGAS-STING signaling pathway. Funding: Government Support – Non-U.S.Fig.1. hUC-MSCs suppress HG induced PF,HMrSV5 EMT ,cgas and sting expression.Data are presented as the mean±SD. *P <0.05 vs. Control, #P <0.05 vs. HG.Fig.2. hUC-MSCs suppress HG induced EMT in HMrSV5 by inhibiting cGAS-STING signaling pathway.Data are presented as the mean±SD. *P <0.05 vs. Control, #P <0.05 vs. DMXAA.