Novel Embedded Platform for High-Throughput, Continuous Transepithelial/Transendothelial Electrical Resistance Measurement of Glomerular Filtration Barrier Co-culture Model

Background: Assessing glomerular filtration barrier (GFB) integrity in vitro is critical for understanding kidney disease mechanisms. Transendothelial/epithelial electrical resistance (TEER) is a key indicator of barrier function in cell culture models. However, existing commercial TEER systems often present limitations in cost-effectiveness and lack customization for long-term continuous, high-through put monitoring capabilities. Methods: We developed a cost-effective, customizable Arduino-based TEER measurement system. It enables simultaneous, continuous data acquisition from 12 transwells, facilitating real-time GFB integrity monitoring in a co-culture model. Designed for standalone operation, it features direct data logging to a secure digital card. The custom-designed, incubator-friendly, 3D-printed lid architecture allows closed-lid cell culture systems, ensuring uninterrupted and automated data capture. It incorporates programmable electrical signal processing to eliminate stray electrochemical effects while using platinum electrodes for precise measurements. Results: The developed system allows continuous TEER measurements across 12 wells concurrently, ensuring high signal quality, accuracy and reliability. The platform enables real-time tracking of barrier integrity changes in response to various experimental conditions, like matrix remodelling triggers and barrier breach agents relevant to kidney disease and renal failure studies. The system facilitates long-term experiments with minimal disturbance. Conclusion: This novel, customizable Arduino-based TEER system offers a significant advantage for studying the dynamics of GFB integrity in vitro. Its capacity for continuous, simultaneous measurements across multiple wells, coupled with standalone operation and design considerations for minimizing experimental artifacts, provides a powerful tool for investigating kidney disease mechanisms and evaluating potential therapeutic interventions.Schematic of TEER Measurement System