Metabolic engineering of Yarrowia lipolytica for enhanced microbial production of medium-chain α, ω-diols from alkanes via CRISPR-Cas9 mediated pathway optimization and P450 alkane monooxygenase overexpression
This study demonstrates the first successful biotransformation of medium- to long-chain α, ω-diols directly from alkanes in yeast. The rational pathway design and oxidation-pathway blocking highlight Y. lipolytica as a promising cell factory for alkane-based biomanufacturing and lay the groundwork for sustainable production of high-value diol precursors through targeted metabolic engineering.
Authors
- Hyungdon Yun (ORCID: https://orcid.org/0000-0002-9125-975X)
- Won‐Young Choi (ORCID: https://orcid.org/0000-0002-3576-5682)
- Ye Chan Kim (ORCID: https://orcid.org/0000-0001-7874-9618)
- Byung‐Gee Kim (ORCID: https://orcid.org/0000-0002-3776-1001)
- Eun-Jung Kim (ORCID: https://orcid.org/0000-0002-3794-6030)
Institutions
- Seoul National University (KR)
- Konkuk University (KR)
- Io Therapeutics (United States) (US)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-10-23
- DOI
- https://doi.org/10.3389/fbioe.2025.1695661
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Konkuk University