Thymoquinone/β-N-acetylglucosaminidase, a novel plant-derived combination, inhibited quorum sensing signaling pathways and disrupted biofilm in Staphylococcus aureus

The combination of TQ and NAGase is a promising antibiofilm agent in <i>S. aureus</i>, offering several advantages over traditional options. It effectively targets both QS pathways and breaks down polysaccharide intercellular adhesins, in addition to exhibiting antibacterial properties to combat bacteria within existing biofilms. The presence of NAGase, a naturally occurring enzyme in cellular lysosomes, combined with the optimal pharmacokinetic properties of TQ, makes it a potential treatment for systemic and localized <i>S. aureus</i> infections.