Anti-ALDOA antibody: a novel diagnostic-associated autoantibody in myasthenia gravis
This study identified novel autoantibodies in patients with seronegative myasthenia gravis (SNMG).Autoantibodies in SNMG sera were screened using a targeted binding assay (TBA). The target antigen was identified via immunoprecipitation and mass spectrometry, and validated by western blotting using a commercial antigen. A cell-based assay (CBA) employing HEK293 cells expressing the identified antigen was established to detect specific antibodies in 676 MG patients and 20 controls.Aldolase A (ALDOA) was identified as a novel autoantigen. Anti-ALDOA antibodies (ALDOA-Ab) demonstrated high diagnostic specificity in SNMG and were also detected in seropositive MG patients, with positivity rates of 4.89% in AChR-Ab' and 2.86% in MuSK-Ab' subgroups. ALDOA-Ab was predominantly of the IgG1 subclass. ALDOA-Ab may serve as a potential diagnostic biomarker for MG. Further studies are needed to investigate its pathogenic role.
Authors
- Xinyue Zhou (ORCID: https://orcid.org/0000-0002-3242-1518)
- Feng Gao (ORCID: https://orcid.org/0000-0002-4961-3495)
- Zhe Ruan (ORCID: https://orcid.org/0000-0002-8190-4022)
- Jia Hu (ORCID: https://orcid.org/0000-0001-5779-257X)
- Ting Chang (ORCID: https://orcid.org/0000-0002-7546-8017)
- Xue Zhao (ORCID: https://orcid.org/0000-0003-4297-8923)
- Yingna Zhang (ORCID: https://orcid.org/0000-0002-8746-5788)
- Qian Liu (ORCID: https://orcid.org/0000-0002-0531-1321)
- Jing Zhang (ORCID: https://orcid.org/0000-0002-7346-555X)
- Peipei Liu (ORCID: https://orcid.org/0000-0002-9097-3425)
- Mengdi Zhang (ORCID: https://orcid.org/0000-0001-5752-9464)
- Junhong Yang
- Quanli Gao (ORCID: https://orcid.org/0000-0002-9232-1623)
- Ruichen Liu (ORCID: https://orcid.org/0000-0001-9362-0580)
- Haiyan Dong
- Jie Lv (ORCID: https://orcid.org/0000-0003-2329-4817)
- Jing Liu (ORCID: https://orcid.org/0000-0002-3255-3019)
Institutions
- Zhengzhou University (CN)
- First Affiliated Hospital of Henan University (CN)
- Air Force Medical University (CN)
Publication Details
- Journal
- Frontiers in Neurology
- Published
- 2025-10-23
- DOI
- https://doi.org/10.3389/fneur.2025.1696583
- Primary Topic
- Myasthenia Gravis and Thymoma
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Academy of Pharmaceutical Sciences
- National Natural Science Foundation of China