Sea Cucumber (Isostichopus badionotus): Bioactivity and Wound Healing Capacity In Vitro of Small Peptide Isolates from Digests of Whole-Body Wall or Purified Collagen
Low-molecular-weight peptides derived from the digestion of body wall proteins in some sea cucumber species have wound-healing and health-promoting properties, but their potency varies widely by species, growth environment, age, and season. For the first time, small peptide (1-3 kDa) fractions have been isolated from the whole-body wall of the sea cucumber <i>Isostichopus badionotus</i> and its constituent collagen and tested for wound healing capacity in vitro. Ultrafiltered digests (1-3 kDa) of the pure collagen, as well as 1-3 kDa digests of the whole-body wall, had potent antioxidant activities and promoted rapid wound healing in a keratinocyte scratch wound assay. Gene expression studies suggested that the wound-healing actions of the individual collagen and body wall 1-3 kDa fractions differed significantly. Low-molecular-weight peptides derived from <i>I. badionotus</i> collagen did promote wound healing in vitro; however, their efficacy may have been modulated by additional factors produced during body wall or collagen digestion. These findings need to be confirmed in vivo.
Authors
- Rossanna Rodríguez‐Canul (ORCID: https://orcid.org/0000-0003-0469-1489)
- Rosa Moo‐Puc (ORCID: https://orcid.org/0000-0001-9204-6062)
- Leticia Olivera‐Castillo
- Sergio Rodrı́guez-Morales (ORCID: https://orcid.org/0000-0002-9788-2403)
- Miguel Á. Olvera‐Novoa (ORCID: https://orcid.org/0000-0002-8304-3668)
- Ariadnna Cruz‐Córdova (ORCID: https://orcid.org/0000-0001-8018-1275)
- Honorio Cruz‐López (ORCID: https://orcid.org/0000-0001-9736-0288)
- Jonatan Jafet Uuh Narváez (ORCID: https://orcid.org/0000-0001-9853-5617)
- Leydi Maribel Carrillo‐Cocom (ORCID: https://orcid.org/0000-0002-5477-7603)
- George Grant (ORCID: https://orcid.org/0000-0002-6579-2331)
- César Puerto-Castillo
- Óscar Medina‐Contreras (ORCID: https://orcid.org/0000-0002-4432-7780)
- Juan Valerio Cauich‐Rodríguez (ORCID: https://orcid.org/0000-0003-0809-7794)
- D. Alejandro Fernández‐Velasco (ORCID: https://orcid.org/0000-0003-2368-6378)
- Frank Segura-Cadiz (ORCID: https://orcid.org/0000-0002-6487-0000)
- Gabriela de Jesus Moo-Pech
Institutions
- University of Aberdeen (GB)
- Autonomous University of Yucatán (MX)
- Consejo Nacional de Ciencia y Tecnología (PY)
- Hospital Infantil de México Federico Gómez (MX)
- Secretaría de Investigación, Innovación y Educación Superior (MX)
- Hospital Regional de Alta Especialidad del Bajío (MX)
- Instituto Politécnico Nacional (MX)
- Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (MX)
- Universidad Nacional Autónoma de México (MX)
Publication Details
- Journal
- Marine Drugs
- Published
- 2025-10-22
- DOI
- https://doi.org/10.3390/md23110411
- Primary Topic
- Protein Hydrolysis and Bioactive Peptides
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional