SUN-588 Association between Vitamin B 12 deficiency and Metformin use among patients with Type 2 Diabetes Mellitus: a cross-sectional analytical study

Abstract Disclosure: A.E. Perez: None. O.C. Dampil: None. Background: Long-term metformin use has been linked to vitamin B12 deficiency. Studies report varying prevalence rates.Data on the Filipino population remains lacking. This study aimed to determine the associationand risk factors between metformin use and vitamin B12 deficiency among Filipino T2DM patients. Methodology: This is a cross-sectional study which involved T2DM patients on Metformin screened for Vitamin B12 deficiency. Shapiro-Wilk test assessed normality, and vitamin B12 deficiency prevalence was computed. Logistic regression estimated odds ratios for the association between metformin use and B12 deficiency, while Spearman correlation assessed the relationship between B12 levels and metformin dose duration. Result: In this study of 369 patients with T2DM on Metformin, the prevalence of Vitamin B12 deficiency was 5.69%.Among the risk factors analyzed, only Hyperthyroidism was significantly associated with Vitamin B12 deficiency, with patients being over eight times more likely to have Vitamin B12 deficiency compared to those without hyperthyroidism (p = 0.025). In contrast, sulfonylurea use was associated with a significantly reduced risk, lowering the odds of Vitamin B12 deficiency by 89% (p = 0.034). A weak negative correlation was observed between Vitamin B12 levels and the total daily dosage of Metformin (p < 0.001) as well as the dose-duration product (p < 0.001). These findings suggest that higher daily doses and cumulative exposure to Metformin are linked to lower Vitamin B12 levels. Patients with Vitamin B12 deficiency had a significantly higher median dose-duration product compared to those with normal Vitamin B12 levels (p = 0.036), reinforcing the role of cumulative Metformin exposure in Vitamin B12 depletion. Conclusion: The prevalence of vitamin B12 deficiency among Filipino patients with T2DM on Metformin is 5.69%. There is a weak negative correlation between Vitamin B12 levels and both daily Metformin dose and cumulative exposure suggests that prolonged and higher doses contribute to vitamin B12 deficiency.Hyperthyroidism was the only significant risk factor identified, increasing the likelihood of deficiency by more than eightfold. While sulfonylurea use was associated with a significantly lower risk. Presentation: Sunday, July 13, 2025