SAT-239 Sex-specific Effects Of Tdcpp Exposure On Body Composition And Glucose Metabolism In Mice Housed Under Thermoneutral Condition

Abstract Disclosure: K. Oriakhi: None. K. Sewell: None. C.S. Rashid: None. Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is a widely used organophosphate flame retardant that has been increasingly linked to adverse metabolic effects. This study investigated the impact of TDCPP exposure on body composition and glucose metabolism in male and female C57BL/6J mice housed under thermoneutral (TNT) conditions. Mice were fed a TDCPP-containing diet at a dose of 1 mg/kg/day ad libitum for 8 weeks. Body weight and composition, including percent fat (%fat) and percent lean mass (%lean), were assessed using EchoMRI, while glucose tolerance and insulin sensitivity were evaluated using intraperitoneal glucose tolerance tests (IPGTT) and insulin tolerance tests (IPITT), respectively. In male mice, TDCPP exposure resulted in increased body weight relative to controls. There were no significant differences in %fat or fat-to-lean mass ratio between TDCPP-exposed and control males housed at either TNT or room temperature. However, %lean mass was significantly reduced in TDCPP-exposed males housed at room temperature compared to those housed at TNT. Additionally, TDCPP-exposed males exhibited impaired glucose tolerance, evidenced by elevated blood glucose levels and increased area under the curve (AUC) during IPGTT in TNT-housed mice. Insulin sensitivity, as assessed by IPITT, showed a modest but non-significant reduction in exposed males. In contrast, female mice showed no significant differences in body weight, %fat, or %lean mass between TDCPP-exposed and control groups. Glucose and insulin tolerance were not impaired in exposed females, and IPITT revealed no meaningful changes in insulin sensitivity. Overall, these findings suggest that TDCPP exposure induces sex-specific metabolic alterations, with male mice exhibiting increased body weight and impaired glucose regulation, while female mice appear relatively unaffected under similar exposure conditions. Presentation: Saturday, July 12, 2025