MON-143 Point-of-care Microscale Magnetic Resonance Effectively Monitors Oxidative Stress Mitigation In Preconception Women With Obesity Undergoing Digital Health Intervention

Abstract Disclosure: R. Rohr: LarmorBIO. A.T. Alves: LarmorBIO. M.J. McPhaul: LarmorBIO. C. Ku: None. Y. Lee: None. F. Yap: None. J. Chan: None. L. Ling: None. B.O. Boehm: LarmorBIO. Obesity poses a significant risk factor for adverse pregnancy and neonatal outcomes, with pre-pregnancy obesity independently contributing to maternal and fetal complications. Oxidative stress is closely linked to obesity and pregnancy complications, however, tests are not currently available in clinical settings. Microscale Magnetic Resonance (MMR - LarmorBio, Waltham MA) has been validated as a sensitive and real-time biomarker of oxidative stress, previously demonstrating responsiveness to GLP-1 treatment1. To enhance risk stratification and support personalized digital health treatment strategies we used MMR to assess oxidative stress of preconception women with obesity enrolled in the Healthy Early Life Moments in Singapore (HELMS)2 study. This longitudinal intervention trial investigates whether an integrated lifestyle intervention, initiated at preconception, sustained through pregnancy and postpartum, augmented by app-based digital health tools, can improve metabolic and mental health in women with overweight and obesity ([BMI] 25-40 kg/m²). We assessed fasting plasma oxidative stress in 54 women followed while trying to conceive at baseline (PC1), 6 months (PC3), and 12 months (PC5) during preconception. MMR revealed a significant reduction in oxidative stress over one year: PC1 oxidative stress levels were the highest at 2.83 a.u. (SD 0.27), PC3 levels decreased to 2.67 a.u. (SD 0.24; p<0.001) and by PC5, levels further reduced to 2.54 a.u. (SD 0.19; p<0.05), reflecting an overall 11% reduction in oxidative stress (p<0.001). No significant changes were observed in BMI, cholesterol levels, fasting plasma glucose, HbA1c, insulin, and C-peptide for the same time period. Oxidative stress showed significant correlations with hematological markers (MCV: r = -0.23, p < 0.001; MCH: r = -0.20, p < 0.001) and metabolic markers (fasting blood glucose: r = 0.1, p < 0.05; BMI: r = 0.14, p < 0.001; percent body fat: r = 0.18, p < 0.001; fasting insulin: r = 0.17, p < 0.001). Additionally, higher oxidative stress was correlated to insulin resistance (HOMA-IR: r = 0.17, p < 0.001) and increased monocyte count(r= 0.17 p < 0.001). Our preliminary findings highlight MMR as a sensitive tool for monitoring oxidative stress in response to digital health interventions. Providing a dynamic indicator of physiological changes in metabolic health beyond conventional biomarkers facilitating personalized assessment and treatment. 1- Journal of the Endocrine Society, Volume 8, Issue Supplement_1, October-November 2024, bvae163.882, https://doi.org/10.1210/jendso/bvae163.882 2 - BMJ Open. 2022 Dec 12;12(12):e061556. doi: 10.1136/bmjopen-2022-061556. PMID: 36523242; PMCID: PMC9748919. Presentation: Monday, July 14, 2025