OR33-06 REGENERATIVE NANOFAT MEMBRANE IN DIABETIC FOOT, A BIOLOGICAL DRESSING

Abstract Disclosure: P. Martin-Marfil: None. N. Fakih-Gomez: None. Introduction: Diabetic foot ulcers (DFUs) are a prevalent complication worldwide, contributing to substantial morbidity, reduced quality of life, and high healthcare expenditures. Traditional treatment options often produce inconsistent healing outcomes, underscoring the demand for innovative therapeutic approaches. Advances in regenerative medicine, particularly Platelet-Rich Fibrin (PRF) and nanofat grafting, have demonstrated potential in promoting tissue repair. This study investigates the creation and application of a novel nanofat-based membrane incorporating PRF for the management of chronic DFUs. Methods: Between April 2019 and April 2024, a total of 129 patients with chronic, non-healing DFUs underwent treatment with a nanofat membrane. The nanofat was derived through mechanical emulsification of adipose tissue, while PRF was obtained by centrifuging autologous blood. These components were combined using the Fakih-Manay Fat Membrane Device to generate a biocompatible membrane, which was subsequently applied to the ulcer site. Patients were monitored bi-weekly for the first month and then on a monthly basis until full wound closure was achieved. Results: The use of the nanofat membrane significantly accelerated wound healing, with ulcer sizes ranging from 2 cm × 3 cm to 9 cm × 9 cm. Complete epithelialization occurred within 1 to 6 months, depending on wound dimensions. The study cohort comprised predominantly female patients (85%) with a mean age of 41 years. Additionally, 31% of participants presented with active infections and received concurrent antibiotic therapy. High levels of patient satisfaction were reported, with notable improvements in wound closure and no recorded complications during follow-up. Conclusion: The integration of nanofat and PRF into a structured membrane offers a promising and effective strategy for managing chronic DFUs. By harnessing the regenerative capacity of adipose-derived stem cells and PRF growth factors, this method enhances wound healing and may reduce the necessity for secondary surgical interventions. Further studies with larger patient populations are required to confirm these findings and broaden the clinical applicability of this technique. Presentation: Monday, July 14, 2025