Development and Validation of a Lectin-independent Liquid Chromatography–Tandem Mass Spectrometry Method for Serum Glycosylated Alpha-fetoprotein Analysis and Comparison with a Liquid-phase Binding Assay
The validated LC-MS/MS method demonstrated robust analytical performance and superior diagnostic accuracy over LiBA for HCC diagnosis while avoiding the inherent limitations of lectin-based assays. Our LC-MS/MS assay shows promise for early HCC detection and may contribute to enhanced patient care.
Authors
- Dohsik Minn (ORCID: https://orcid.org/0000-0002-5794-9714)
- Jae Youn Cheong (ORCID: https://orcid.org/0000-0001-6246-1783)
- J. H. Park
- Saeyoung Lee (ORCID: https://orcid.org/0000-0001-8901-799X)
- Je‐Hyun Baek (ORCID: https://orcid.org/0000-0003-4974-8397)
- H. S. Suh
- Soon Sun Kim (ORCID: https://orcid.org/0000-0002-6862-1896)
- H. Y. Kim
- Won Suk Yang (ORCID: https://orcid.org/0000-0002-2323-6652)
- Y.-S. Park (ORCID: https://orcid.org/0009-0003-7411-9338)
Institutions
- Korea Institute of Radiological and Medical Sciences (KR)
- Ajou University (KR)
Publication Details
- Journal
- Annals of Laboratory Medicine
- Published
- 2025-10-23
- DOI
- https://doi.org/10.3343/alm.2025.0003
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00