Two-point calibration protocol for the Förster Resonance Energy Transfer indicator Pyronic in neurons
The FRET-based fluorescent indicator Pyronic can be reliably calibrated using a minimally invasive, pharmacology-based two-point calibration protocol in neurons, thus providing a robust and quantitative method to study pyruvate metabolism under various physiological and pathological scenarios.
Authors
- Felipe Baeza‐Lehnert (ORCID: https://orcid.org/0000-0001-8026-2147)
- Camila Aburto
- Alejandro San Martín (ORCID: https://orcid.org/0000-0002-5608-5117)
- Yasna Contreras-Baeza
Institutions
- Centro de Estudios Científicos (CL)
- Leipzig University (DE)
Publication Details
- Journal
- Neurophotonics
- Published
- 2025-10-22
- DOI
- https://doi.org/10.1117/1.nph.12.s2.s22807
- Primary Topic
- Neuroscience and Neural Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agencia Nacional de Investigación y Desarrollo