#2212 Atopic dermatitis-like chronic inflammation may be associated with kidney inflammation

Abstract Background and Aims Some studies recently indicated the association between renal abnormalities and atopic dermatitis (AD). AD causes not only skin lesions, but also the dysregulation of a wide range of cytokines. However, the underlying mechanism for a potential link between AD and chronic kidney diseases remains unknown. Method To evaluate the status of kidney functions of patients with AD, the serum and urine samples of US adults from the National Health and Nutrition Examination Survey (NHANES) were analyzed. Additionally, a chronic AD-like mouse model was induced by applying 2,4-dinitrochlorobenzene. The renal function was evaluated by urine and serum examinations, histological staining and electron microscopy imaging of kidneys. The expression of glomerular filtration barrier proteins was evaluated by immunoblotting and immunofluorescence staining. Furthermore, real-time PCR and ELISA assays were performed to analyze kidney inflammation. Results Analysis of NHANES data confirmed the upregulation of urine protein creatinine ratio and downregulation of estimate GFR in patients with AD. In addition, AD-like mice showed higher albuminuria and serum creatinine than control mice. Moreover, AD mice had an increase in glomerular cell infiltration, and foot process effacement of podocytes, indicating dysfunction of glomerular filtration by AD-like inflammation. Kidneys from AD mice showed downregulated expression of glomerular filtration barrier proteins. Concurrently, S100A8 and S100A9, which are the Ca2+ binding proteins derived from neutrophils and macrophages, were found to be elevated in the serum and renal cortex of these AD mice. Conclusion Our findings suggest that dysfunctional glomerular filtration and reduced kidney function in AD-like mice might be correlated with the increased levels of S100A8 and A100A9.