Temperature‐Dependent Stability of Components in a Particle‐based Continuous Biosensor

Abstract Biosensing by Particle Motion (BPM) is an affinity‐based biosensing technology for the continuous monitoring of concentrations of specific biomolecules. Previous work has shown that BPM sensor signals change over long time spans, caused in part by losses of binder molecules from the particles and the sensing surface. This paper studies whether the aging effects of particles and the sensing surface depend on the applied temperature. Particles and surface were separately aged, and their functionalities were quantified in a glycoalkaloid BPM sensor, with anti ‐solanidine antibodies on the particles and solanidine‐analogue on the sensing surface. The results show that the particles are stable up to 40 °C and lose functionality when exposed to 50 °C and higher. The surface aging experiments show both decreasing and increasing signals with temperature exposures, potentially caused by rearrangements of the low‐fouling polymer coating on the sensing surface. The experimental results give pointers on how long‐term changes of continuous biosensors can be studied and eventually mitigated.