Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions

In Experiment 1, significant differences in recovery were observed across products and volumes (p < 0.05), with paper products 3 and 4 releasing the highest volumes. In Experiment 2, linear regression analysis showed that paper type, elution buffer, virus dilution, and their interactions affected viral RNA recovery (p < 0.05). AUC analysis showed no significant difference in PRRSV RNA detection between buffer-specific positive controls and product 3 eluted with lysis buffer or water. Similarly, no difference was detected in PEDV RNA detection between the positive control eluted with lysis buffer and products 3 and 4 eluted with lysis buffer. These results demonstrated that the choice of filter paper and the procedures used for viral RNA detection significantly affect target recovery.