Decoding the Genome of the <i>Komagataella phaffii</i> Type Strain CBS 2612
The methylotrophic yeast Komagataella phaffii (formerly known as Pichia pastoris) is an essential host for biotechnological production. Here, we present the complete and annotated genome sequence of CBS 2612 T = NRRL Y-7556T, the type strain of K. phaffii. CBS 2612 has a genome length of 9,387,549 bp with 5412 predicted and 5389 annotated genes, of which 144 are tRNA genes including the previously missing tRNAs for tryptophan, tyrosine, and serine, and 34 rRNA genes. In total, 4 SNPs were found compared to the biotechnologically most commonly used strain, CBS 7435. Additionally, 34 lncRNA candidates could be identified, including candidates that affect telomere-regulation and flocculin genes.
Authors
- Minoska Valli
- Lina Heistinger (ORCID: https://orcid.org/0000-0002-0935-4652)
- Diethard Mattanovich (ORCID: https://orcid.org/0000-0002-0907-4167)
- Brigitte Gasser (ORCID: https://orcid.org/0000-0003-2881-6370)
- Alexandra B. Graf (ORCID: https://orcid.org/0000-0002-2738-3270)
- Nadine E. Tatto (ORCID: https://orcid.org/0000-0002-6989-6744)
- Corinna Rebnegger
Institutions
- Hochschule Campus Wien (AT)
- Pädagogische Hochschule Wien (AT)
- Christian Doppler Laboratory for Thermoelectricity (AT)
- Austrian Centre of Industrial Biotechnology (Austria) (AT)
- BOKU University (AT)
Publication Details
- Journal
- Yeast
- Published
- 2025-10-21
- DOI
- https://doi.org/10.1002/yea.70004
- Primary Topic
- Fungal and yeast genetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Amt der NÖ Landesregierung
- Österreichische Forschungsförderungsgesellschaft
- Christian Doppler Forschungsgesellschaft
- Universität für Bodenkultur Wien
- Steirische Wirtschaftsförderungsgesellschaft
- Standortagentur Tirol