A practical approach to panel design, validation, and interpretation for the evaluation of <scp>T</scp> ‐cell neoplasms by flow cytometry

The diagnosis of T-cell neoplasms remains one of the most challenging areas in hematopathology due to the immunophenotypic heterogeneity and subtle aberrancies often present in these entities. This "Best Practice" manuscript provides a practical framework for laboratories to design, validate, and interpret immunophenotyping studies of immature and mature T-cell neoplasms. We outline the utility of key antigens in the screening and classification of T-cell lymphomas/leukemia including TRBC1 and TRBC2. Analytical strategies using the "difference from normal" method and template-based gating are discussed, along with validation considerations aligned with CLSI H62 guidelines. By integrating these principles into laboratory workflows, this manuscript aims to standardize and improve the assessment of T-cell neoplasms across diverse clinical settings.

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Publication Details

Journal
Cytometry Part B Clinical Cytometry
Published
2025-10-22
DOI
https://doi.org/10.1002/cyto.b.22258
Citations
3
Primary Topic
T-cell and Retrovirus Studies
Type
article
Field-Weighted Citation Impact
2.39
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article

A practical approach to panel design, validation, and interpretation for the evaluation of T ‐cell neoplasms by flow cytometry

Lianqun Qiu, Katherine Devitt, Jonathan R. Fromm, Ahmad Al‐Attar et al.
3 citations
Cytometry Part B Clinical Cytometry
T-cell and Retrovirus Studies
2.39
article

A practical approach to panel design, validation, and interpretation for the evaluation of T ‐cell neoplasms by flow cytometry

Lianqun Qiu, Katherine Devitt, Jonathan R. Fromm, Ahmad Al‐Attar, Jolene Cardinali, Min Shi, Wolfgang Kern, Andrea Illingworth, Felipe Magalhaes Furtado, Pedro Horna, Jean S. Oak
article en
3 citations

Abstract

The diagnosis of T-cell neoplasms remains one of the most challenging areas in hematopathology due to the immunophenotypic heterogeneity and subtle aberrancies often present in these entities. This "Best Practice" manuscript provides a practical framework for laboratories to design, validate, and interpret immunophenotyping studies of immature and mature T-cell neoplasms. We outline the utility of key antigens in the screening and classification of T-cell lymphomas/leukemia including TRBC1 and TRBC2. Analytical strategies using the "difference from normal" method and template-based gating are discussed, along with validation considerations aligned with CLSI H62 guidelines. By integrating these principles into laboratory workflows, this manuscript aims to standardize and improve the assessment of T-cell neoplasms across diverse clinical settings.

Cytometry Part B Clinical CytometryVol. 108(6)
Hartford Hospital (US), Mayo Clinic (US), The University of Texas MD Anderson Cancer Center (US), University of Pittsburgh (US), University of Washington (US), University of Vermont Medical Center (US), Munich Leukemia Laboratory (Germany) (DE), Sabin Medicina Diagnostica (Brazil) (BR), Hospital Universitário de Brasília (BR), CS Diagnostics (DE), Stanford University (US)
Openalex Percentile: Top 10%
T-cell and Retrovirus Studies
2.39
DOIOpen Access PDFOpenAlex
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