Real-time, high-throughput super-resolution microscopy via panoramic integration
We introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized line-scan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity. This versatile platform offers a practical pathway toward biological insights beyond traditional optical and computational constraints.
Authors
- Kyungduck Yoon (ORCID: https://orcid.org/0000-0002-3106-9735)
- William C. Ratcliff (ORCID: https://orcid.org/0000-0002-6837-8355)
- Anthony Burnetti (ORCID: https://orcid.org/0000-0003-3856-8251)
- Kidan Tadesse
- Biagio Mandracchia (ORCID: https://orcid.org/0000-0002-4583-4733)
- Shu Jia (ORCID: https://orcid.org/0000-0001-6854-7537)
- Sayantan Datta (ORCID: https://orcid.org/0000-0002-0529-4402)
- G. Ozan Bozdag (ORCID: https://orcid.org/0000-0002-0931-2399)
- Hansol Yoon (ORCID: https://orcid.org/0000-0002-3410-927X)
- Zhi Ling (ORCID: https://orcid.org/0000-0002-3083-6031)
Institutions
- Georgia Institute of Technology (US)
- The Wallace H. Coulter Department of Biomedical Engineering (US)
- Quantitative BioSciences (US)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-10-21
- DOI
- https://doi.org/10.1038/s41467-025-64368-0
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00