The Phytohormone Signaling Pathway and Immunity Responses to BYDV Infection in Resistant and Susceptible Oat Cultivars

Oat (<i>Avena sativa</i> L.) is a vital cereal and feed crop grown worldwide, but its production is increasingly threatened by barley yellow dwarf virus (BYDV) and aphid infestations in arid and semi-arid regions, particularly in northern China. This study explores the transcriptomic and physiological responses of two oat cultivars MN10253 (resistant) and Qingyin 1 (susceptible) to BYDV at 0, 2, 8, 24, and 48 h post-infection. A combination of phytohormone profiling, differential gene expression analysis, and pathway enrichment was employed to identify mechanisms underpinning disease resistance. Comparative time-course transcriptome analysis revealed 9285 and 8904 differentially expressed genes (DEGs) in MN10253 and Qingyin 1, respectively. Key pathways such as MAPK signaling, plant-pathogen interaction, and hormone signal transduction were significantly enriched. The resistant cultivar exhibited robust activation of pattern-triggered immunity and effector-triggered immunity pathways, marked by upregulation of genes like <i>RPS2</i>, <i>HSP90</i>, and <i>WRKY33</i>, alongside higher expression of salicylic acid (SA)-responsive genes, such as <i>NPR1</i> and <i>PAL.</i> Conversely, the susceptible cultivar displayed weaker or delayed activation of these defense pathways. Hormonal analysis further demonstrated higher SA accumulation in MN10253 during early infection, correlating with enhanced defense responses. In contrast, Qingyin 1 showed elevated levels of auxin and abscisic acid, which are linked to suppressed immunity. This study underscores the central role of immunity responses and phytohormone pathways in mediating oat resistance to BYDV, highlighting the tradeoff between growth and defense modulated by hormonal crosstalk. These findings advance our understanding of host-pathogen dynamics in oats and provide valuable insights for breeding disease-resistant cultivars.