Low-cost luminescence lifetime system for medical diagnostics

Abstract Luminescence lifetimes provide insights into the local microenvironment of luminescent molecules, enabling precise measurements that can be leveraged for biomedical imaging and sensing applications. Traditional systems for measuring luminescence lifetimes rely on expensive ultrafast detectors and electronics. Here, a novel optical luminescence lifetime estimation method, modular analysis and eXtraction system for affordable lifetime imaging of fluorescence signals (MAX-alif), was developed to provide a high-speed, low-cost, and scalable solution for luminescence lifetime measurements. In MAX-alif, excitation light with sinusoidal intensity modulation is combined with synthetic path-length difference analysis and machine learning regression to compute luminescence lifetimes from intensity image features. An acousto-optical modulator within the detection path transfers the synthesized phase difference between the excitation and emission light into intensity differences that a standard camera can capture. Both simulation and experimental data were used to generate and validate models for the calibration and extraction of lifetime values from intensity datasets. MAX-alif measurements of phosphorescent and fluorescent samples achieved mean and median lifetime values within 5% of the reference lifetime values. By eliminating the need for expensive hardware and enabling accurate lifetime imaging with standard cameras, MAX-alif allows widespread, cost-effective luminescence lifetime imaging for medical diagnostics applications.