Development of Analytical Screening Method for Rapid Identification of Carica Papaya Seed Adulterants in Black Pepper

Adulteration is a critical issue that undermines the credibility and therapeutic potential of Ayurvedic medicine. Ensuring authenticity through scientific methods and regulatory oversight is essential to preserve the heritage and promote global acceptance of Ayurveda. With regards to above concern HPLC and HPTLC method has been established for identification of adulteration of Carica papaya seed in black pepper. The methanolic extracts of Carica papaya seeds, along with authenticated samples of Piper nigrum and an adulterated mixture, were simultaneously applied onto the TLC plate. After the development, the plates were scanned at a wavelength of 254 nm. The HPLC analysis showing specific Rt at 28.1 min. for the specific marker present in Carica papaya seeds and resulting chromatogram of HPTLC revealed a distinct Rf value of 0.69 in both the Carica papaya seed extract and the adulterated mixture track, thereby proving the presence of papaya seed in the adulterated sample. This method offers a reliable approach for rapid identification of Carica papaya seed adulteration in black pepper.