Exosomes derived from Schwann cells (SCDE) promote tendon repair through the PTEN/PI3K/Akt signaling pathway

Tendon injury is one of the most frequently encountered soft tissue injuries with slow repair and poor functional recovery. Over the past few years, there's been a buzz about how exosomes might boost tissue repair and regeneration. This research dive delves into the question of whether Schwann cell-derived exosomes (SCDEs) can jumpstart tendon healing by tweaking the PTEN/PI3K/Akt signaling chain.been concentrated on the effects of exosomes in promoting tissue regeneration and repair. In the present study, we would like to explore whether exosomes derived from Schwann cells (SCDE) could promote tendon repair via modulation of PTEN/PI3K/Akt signaling cascade. This study integrates experiments performed both in vitro and in vivo. Tendon cells were categorized into the NC and SCDE groups, with a scratch assay employed.vitro and in vivo conditions tendo cells were divided into the NC group and SCDE group, and scratch assay, Transwell migration assay, flow cytometry for cell cycle detection, and Western blot and qRT-PCR analyses were performed to measure SCX, DCN, COL1A1, p-AKT, PTEN, and other markers.In the in vivo experiments, a rat model of 1/3 patellar tendon defect was established, and hydrogel or hydrogel + SCDE was injected. Tissues were gathered at 2, 4, and 8 weeks following the surgical procedure, and HE, Masson, and Sirius Red stains were applied to gauge the healing process. Important protein levels were assessed at the 2 week mark after the operation.Masson, and Sirius Red staining were performed to evaluate tissue repair. Key protein expression was measured at 2 weeks post-surgery.