Multiplex long-amplicon sequencing for comprehensive molecular surveillance of Plasmodium falciparum resistance to artemisinin and partner drugs in artemisinin-based combination therapies (ACTs)
The new long-amplicon panel improves malaria molecular drug-resistance monitoring by detecting known and emerging mutations across entire genes in a single test. The high sensitivity for ultralow parasitemia samples, coupled with their species specificity and cost-effectiveness, makes it a scalable tool for monitoring multidrug-resistant P. falciparum strains, particularly in resource-limited settings.
Authors
- Yinlong Wang (ORCID: https://orcid.org/0000-0002-1570-9026)
- Yaobao Liu (ORCID: https://orcid.org/0000-0001-9616-8948)
- Guoding Zhu
- Jiayao Zhang (ORCID: https://orcid.org/0000-0001-5320-9009)
- Jun Cao (ORCID: https://orcid.org/0000-0002-6894-4011)
- Zhiyi Mao
- Sui Xu
- Fei Li (ORCID: https://orcid.org/0000-0001-6211-6306)
Institutions
- Jiangsu Institute of Parasitic Diseases (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2025-10-21
- DOI
- https://doi.org/10.1186/s13071-025-07058-6
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China